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SUR-8, a conserved Ras-binding protein w ... mediated signaling in C elegans
SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans.
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We describe the identification and characterization of a novel gene, sur-8, that positively regulates Ras-mediated signal transduction during C. elegans vulval development. Reduction of sur-8 function suppresses an activated ras mutation and dramatically enhances phenotypes of mpk-1 MAP kinase and ksr-1 mutations, while increase of sur-8 dosage enhances an activated ras mutation. sur-8 appears to act downstream of or in parallel to ras but upstream of raf. sur-8 encodes a conserved protein that is composed predominantly of leucine-rich repeats. The SUR-8 protein interacts directly with Ras but not with the Ras(P34G) mutant protein, suggesting that SUR-8 may mediate its effects through Ras binding. A structural and functional SUR-8 homolog in humans specifically binds K-Ras and N-Ras but not H-Ras in vitro.
Sieburth DS, Sun Q, Han M
Cell
1998-07-10 00:00
94
1
119-30
Adaptor Proteins, Signal Transducing,Amino Acid Sequence,Animals,Caenorhabditis elegans,Caenorhabditis elegans Proteins,Carrier Proteins,Conserved Sequence,Embryonic Induction,Female,Gene Dosage,Genes, Helminth,Leucine,Mammals,Molecular Sequence Data,Mutation,Phenotype,Protein Binding,Protein Kinases,Protein-Serine-Threonine Kinases,Proto-Oncogene Proteins c-raf,Repetitive Sequences, Nucleic Acid,Sequence Homology, Amino Acid,Signal Transduction,Suppression, Genetic,Vulva,ras Proteins,Adaptor Proteins, Signal Transducing,Caenorhabditis elegans Proteins,Carrier Proteins,SUR-8 protein, C elegans,let-60 protein, C elegans,Leucine,KSR-1 protein kinase,mpk-1 protein, C elegans,Protein Kinases,Protein-Serine-Threonine Kinases,Proto-Oncogene Proteins c-raf,ras Proteins
Howard Hughes Medical Institute, Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, 80309-0347, USA
Cell
NIGMS GM47869
0092-8674
S0092-8674(00)81227-1
511
True
9674433
