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Role of ANC-1 in tethering nuclei to the actin cytoskeleton
Role of ANC-1 in tethering nuclei to the actin cytoskeleton.
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Mutations in anc-1 (nuclear anchorage defective) disrupt the positioning of nuclei and mitochondria in Caenorhabditis elegans. ANC-1 is shown to consist of mostly coiled regions with a nuclear envelope localization domain (called the KASH domain) and an actin-binding domain; this structure was conserved with the Drosophila protein Msp-300 and the mammalian Syne proteins. Antibodies against ANC-1 localized cytoplasmically and were enriched at the nuclear periphery in an UNC-84-dependent manner. Overexpression of the KASH domain or the actin-binding domain caused a dominant negative anchorage defect. Thus, ANC-1 may connect nuclei to the cytoskeleton by interacting with UNC-84 at the nuclear envelope and with actin in the cytoplasm.
Starr DA, Han M
Science (New York, N.Y.)
2002-10-11 00:00
298
5592
406-9
Actins,Alleles,Animals,Animals, Genetically Modified,Caenorhabditis elegans,Caenorhabditis elegans Proteins,Cell Nucleus,Cytoplasm,Cytoskeleton,Genes, Helminth,Membrane Glycoproteins,Microfilament Proteins,Mitochondria,Mutation,Nuclear Envelope,Nuclear Proteins,Phenotype,Protein Binding,Protein Structure, Tertiary,Recombinant Fusion Proteins,ANC-1 protein, C elegans,Actins,Caenorhabditis elegans Proteins,Membrane Glycoproteins,Microfilament Proteins,Nuclear Proteins,Recombinant Fusion Proteins,Unc-84 protein, C elegans
Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
Science
1095-9203
10.1126/science.1075119
1075119
508
True
12169658
