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Mutations in cye-1, a Caenorhabditis ele ... control and vulval development
Mutations in cye-1, a Caenorhabditis elegans cyclin E homolog, reveal coordination between cell-cycle control and vulval development.
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We have identified strong loss-of-function mutations in the C. elegans cyclin E gene, cye-1. Mutations in cye-1 lead to the underproliferation of many postembryonic blast lineages as well as defects in fertility and gut-cell endoreduplication. In addition, cye-1 is required maternally, but not zygotically for embryonic development. Our analysis of vulval development in cye-1 mutants suggests that a timing mechanism may control the onset of vulval cell terminal differentiation: once induced, these cells appear to differentiate after a set amount of time, rather than a specific number of division cycles. cye-1 mutants also show an increase in the percentage of vulval precursor cells (VPCs) that adopt vulval cell fates, indicating that cell-cycle length can play a role in the proper patterning of vulval cells. By analyzing cul-1 mutants, we further demonstrate that vulval cell terminal differentiation can be uncoupled from associated changes in vulval cell division planes.
Fay DS, Han M
Development (Cambridge, England)
2000-09-01 00:00
127
18
4049-60
Amino Acid Sequence,Animals,Biological Clocks,Biological Markers,Caenorhabditis elegans,Cell Count,Cell Cycle,Cell Cycle Proteins,Cell Differentiation,Cell Division,Cell Lineage,Cullin Proteins,Cyclin E,DNA Replication,Female,Genes, Helminth,Helminth Proteins,Microscopy, Fluorescence,Molecular Sequence Data,Mutation,Phenotype,RNA, Double-Stranded,Sequence Alignment,Stem Cells,Vulva,Biological Markers,Cell Cycle Proteins,Cullin 1,Cullin Proteins,Cyclin E,Helminth Proteins,RNA, Double-Stranded
Howard Hughes Medical Institute and Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder CO 80309-0347, USA faydalphacoloradoedu
Development
NIGMS F32 GM20029-01, NIGMS GM47869
0950-1991
502
True
10952902
