Document Actions
Lamin-dependent localization of UNC-84, ... ation in Caenorhabditis elegans
Lamin-dependent localization of UNC-84, a protein required for nuclear migration in Caenorhabditis elegans.
17
Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear periphery until late anaphase, similar to known inner nuclear membrane proteins. UNC-84 protein is first detected at the 26-cell stage and thereafter is present in most cells during development and in adults. UNC-84 is properly expressed in unc-83 and anc-1 lines, which have phenotypes similar to unc-84, suggesting that neither the expression nor nuclear envelope localization of UNC-84 depends on UNC-83 or ANC-1 proteins. The envelope localization of Ce-lamin, Ce-emerin, Ce-MAN1, and nucleoporins are unaffected by the loss of UNC-84. UNC-84 is not required for centrosome attachment to the nucleus because centrosomes are localized normally in unc-84 hyp7 cells despite a nuclear migration defect. Models for UNC-84 localization are discussed.
Lee KK, Starr D, Cohen M, Liu J, Han M, Wilson KL, Gruenbaum Y
Molecular biology of the cell
2002-03-01 00:00
13
3
892-901
Animals,Caenorhabditis elegans,Caenorhabditis elegans Proteins,Centrosome,Gene Expression Regulation, Developmental,Helminth Proteins,Lamins,Membrane Glycoproteins,Membrane Proteins,Mitosis,Models, Biological,Nuclear Envelope,Nuclear Pore Complex Proteins,Nuclear Proteins,Recombinant Fusion Proteins,Thymopoietins,Caenorhabditis elegans Proteins,Ce-MAN1 protein, C elegans,Helminth Proteins,Lamins,Membrane Glycoproteins,Membrane Proteins,Nuclear Pore Complex Proteins,Nuclear Proteins,Recombinant Fusion Proteins,Thymopoietins,Unc-84 protein, C elegans,emerin
Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Mol. Biol. Cell
NIGMS F32GM20127, NICHD F32HD08331, NIGMS R01GM48646
1059-1524
10.1091/mbc.01-06-0294
495
True
11907270
