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A RHO GTPase-mediated pathway is require ... ation in Caenorhabditis elegans
A RHO GTPase-mediated pathway is required during P cell migration in Caenorhabditis elegans.
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The Rho family of guanine triphosphate hydrolases controls various cellular processes, including cell migration. We describe here the demonstration of a role for a RhoA GTPase homologue during cell migration in Caenorhabditis elegans. We show that eliminating or reducing rho-1 gene function by using a dominant-negative transgene or dsRNA interference results in a severe defect in migration of hypodermal P cells to a ventral position. Biochemical and genetic data also suggest that unc-73, which encodes a Trio-like guanine nucleotide exchange factor, may act as an activator of rho-1 in the migration process. Mutations in let-502 ROCK, a homologue of a RhoA effector in mammals, also cause defects in P cell migration, suggesting that it may be one of several effectors acting downstream of rho-1 during P cell migration. Finally, we provide evidence to support the idea that other small Rac subfamily small GTPases act redundantly and in parallel to RHO-1 in this specific cell migration event.
Spencer AG, Orita S, Malone CJ, Han M
Proceedings of the National Academy of Sciences of the United States of America
2001-11-06 00:00
98
23
13132-7
Animals,Caenorhabditis elegans,Cell Movement,Guanine Nucleotide Exchange Factors,Guanosine Diphosphate,Guanosine Triphosphate,Mice,Mice, Transgenic,Mutagenesis, Site-Directed,rho GTP-Binding Proteins,Guanine Nucleotide Exchange Factors,Guanosine Diphosphate,Guanosine Triphosphate,rho GTP-Binding Proteins
Howard Hughes Medical Institute, Department of Molecular Cell and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM47869, NICHD HD8653
0027-8424
10.1073/pnas.241504098
241504098
459
True
11687661
