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Systematic evolution of ligands by expon ... bacteriophage T4 DNA polymerase
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
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High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand selection from pools of variant sequences and amplification of the bound species. Multiple rounds exponentially enrich the population for the highest affinity species that can be clonally isolated and characterized. In particular one eight-base region of an RNA that interacts with the T4 DNA polymerase was chosen and randomized. Two different sequences were selected by this procedure from the calculated pool of 65,536 species. One is the wild-type sequence found in the bacteriophage mRNA; one is varied from wild type at four positions. The binding constants of these two RNA's to T4 DNA polymerase are equivalent. These protocols with minimal modification can yield high-affinity ligands for any protein that binds nucleic acids as part of its function; high-affinity ligands could conceivably be developed for any target molecule.
Tuerk C, Gold L
Science (New York, N.Y.)
1990-08-03 00:00
249
4968
505-10
Base Sequence,DNA-Directed DNA Polymerase,Escherichia coli,Evolution,Genes, Viral,Genetic Techniques,Ligands,Models, Genetic,Molecular Sequence Data,Nucleic Acid Conformation,Polymerase Chain Reaction,RNA, Messenger,RNA, Viral,T-Phages,Transcription, Genetic,Ligands,RNA, Messenger,RNA, Viral,DNA-Directed DNA Polymerase
Department of Molecular, Cellular, Developmental Biology, University of Colorado, Boulder 80309
Science
NIGMS GM 19963, NIGMS GM 28685
0036-8075
444
True
2200121
