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Selection of high-affinity RNA ligands t ... synthesis and RNase H activity
Selection of high-affinity RNA ligands to reverse transcriptase: inhibition of cDNA synthesis and RNase H activity.
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Specific, high-affinity RNA ligands to avian myeloblastosis virus and Moloney murine leukemia virus reverse transcriptases were isolated from a combinatorial RNA library using the SELEX (systematic evolution of ligands by exponential enrichment) procedure. The selected RNA ligands bound their respective reverse transcriptases with approximately nanomolar dissociation constants. The ligands did not exhibit primary sequence conservation from selections against different target enzymes. Moreover, the selected ligands competed with the binding of template/primer complex and inhibited both the RNA-dependent DNA polymerase and the RNase H activities of the cognate reverse transcriptase. SELEX can yield both high-affinity and high-specificity oligonucleotide antagonists against specific members of a protein family.
Chen H, Gold L
Biochemistry
1994-07-26 00:00
33
29
8746-56
Avian myeloblastosis virus,Base Sequence,DNA Primers,DNA, Complementary,Hydrogen Bonding,Leukemia Virus, Murine,Ligands,Molecular Sequence Data,Nucleic Acid Conformation,RNA,Reverse Transcriptase Inhibitors,Ribonuclease H, Calf Thymus,DNA Primers,DNA, Complementary,Ligands,Reverse Transcriptase Inhibitors,RNA,Ribonuclease H, Calf Thymus
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309
Biochemistry
NIGMS GM-19963, NIGMS GM-28685
0006-2960
440
True
7518691
