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RNA replication by Q beta replicase a working model
RNA replication by Q beta replicase: a working model.
54
Two classes of RNA ligands that bound to separate, high affinity nucleic acid binding sites on Q beta replicase were previously identified. RNA ligands to the two sites, referred to as site I and site II, were used to investigate the molecular mechanism of RNA replication employed by the four-subunit replicase. Replication inhibition by site I- and site II-specific ligands defined two subsets of replicatable RNAs. When provided with appropriate 3' ends, ligands to either site served as replication templates. UV crosslinking experiments revealed that site I is associated with the S1 subunit, site II with elongation factor Tu, and polymerization with the viral subunit of the holoenzyme. These results provide the framework for a three site model describing template recognition and product strand initiation by Q beta replicase.
Brown D, Gold L
Proceedings of the National Academy of Sciences of the United States of America
1996-10-15 00:00
93
21
11558-62
Base Sequence,Binding Sites,Cross-Linking Reagents,Kinetics,Ligands,Macromolecular Substances,Molecular Sequence Data,Peptide Elongation Factor Tu,Q beta Replicase,RNA,RNA-Directed DNA Polymerase,Recombinant Proteins,Replicon,Templates, Genetic,Ultraviolet Rays,Uridine Triphosphate,Viruses,Cross-Linking Reagents,Ligands,Macromolecular Substances,Recombinant Proteins,Uridine Triphosphate,RNA,Q beta Replicase,RNA-Directed DNA Polymerase,Peptide Elongation Factor Tu
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309, USA
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM19963
0027-8424
433
True
8876174
