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Quantitative analysis of ribosome binding sites in Ecoli
Quantitative analysis of ribosome binding sites in E.coli.
54
185 clones with randomized ribosome binding sites, from position -11 to 0 preceding the coding region of beta-galactosidase, were selected and sequenced. The translational yield of each clone was determined; they varied by more than 3000-fold. Multiple linear regression analysis was used to determine the contribution to translation initiation activity of each base at each position. Features known to be important for translation initiation, such as the initiation codon, the Shine/Dalgarno sequence, the identity of the base at position -3 and the occurrence of alternative ATGs, are all found to be important quantitatively for activity. No other features are found to be of general significance, although the effects of secondary structure can be seen as outliers. A comparison to a large number of natural E.coli translation initiation sites shows the information profile to be qualitatively similar although differing quantitatively. This is probably due to the selection for good translation initiation sites in the natural set compared to the low average activity of the randomized set.
Barrick D, Villanueba K, Childs J, Kalil R, Schneider TD, Lawrence CE, Gold L, Stormo GD
Nucleic acids research
1994-04-11 00:00
22
7
1287-95
Base Sequence,Binding Sites,Cloning, Molecular,Codon,DNA, Bacterial,Escherichia coli,Molecular Sequence Data,Ribosomes,beta-Galactosidase,Codon,DNA, Bacterial,beta-Galactosidase
University of Colorado, Boulder 80309-0347
Nucleic Acids Res.
NIGMS GM28685, NIGMS GM28755
0305-1048
428
True
8165145
