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Phage T4 expression vector protection from proteolysis
Phage T4 expression vector: protection from proteolysis.
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We have developed an efficient method for the expression of heterologous genes during infection by T4, a bacteriophage known to inhibit the proteolytic systems of Escherichia coli. This system enables us to clone genes in a plasmid expression vector and move them readily into T4. We have used lacZ as a reporter gene to show that both plasmid and phage exhibit low basal expression or high-level expression under the control of a T7 promoter. This system promises a possible solution to the problem of degradation and/or toxicity of overproduced proteins.
Singer BS, Gold L
Gene
1991-09-30 00:00
106
1
1-6
Electrophoresis, Polyacrylamide Gel,Escherichia coli,Gene Expression,Genes, Viral,Genetic Vectors,Hydrolysis,Plasmids,Promoter Regions (Genetics),Recombination, Genetic,T-Phages,Transcription, Genetic
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309
Gene
NIGMS GM19963-18
0378-1119
0378-1119(91)90558-S
425
True
1937029
