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Nature of the ribosomal mRNA track analy ... uences and secondary structures
Nature of the ribosomal mRNA track: analysis of ribosome-binding sites containing different sequences and secondary structures.
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The ribosomal mRNA track was investigated by toeprinting 30S ribosomes, in the presence or absence of tRNA, using a variety of different ribosome-binding sites. We found that: (1) the ribosome, by itself, recognizes the mRNA translational initiation site; (2) the ribosomal mRNA track makes extensive contact with mRNA independent of tRNA and the start codon; (3) ribosome-mRNA complexes are less stable than complexes containing tRNA; and (4) toeprinting can be used to analyze the contour of the ribosomal mRNA track, yielding information on its height as well as its length dimension. Examination of several ribosome-binding sites, including those containing very stable secondary structure, indicated that the height of the mRNA track is quite roomy, while the nucleotide distance between the site of Shine-Dalgarno annealing, the P site, and the 3'-edge of the mRNA track is fixed. The data suggest a mechanism for tethering regulatory elements to the ribosome during translation.
Ringquist S, MacDonald M, Gibson T, Gold L
Biochemistry
1993-09-28 00:00
32
38
10254-62
Base Sequence,Binding Sites,DNA Primers,Escherichia coli,Models, Structural,Molecular Sequence Data,Nucleic Acid Conformation,Polymerase Chain Reaction,RNA, Messenger,RNA-Directed DNA Polymerase,Ribosomes,Structure-Activity Relationship,DNA Primers,RNA, Messenger,RNA-Directed DNA Polymerase
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309
Biochemistry
NIGMS GM-28685
0006-2960
421
True
7691171
