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Libraries for genomic SELEX
Libraries for genomic SELEX.
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An increasing number of proteins are being identified that regulate gene expression by binding specific nucleic acidsin vivo. A method termed genomic SELEX facilitates the rapid identification of networks of protein-nucleic acid interactions by identifying within the genomic sequences of an organism the highest affinity sites for any protein of the organism. As with its progenitor, SELEX of random-sequence nucleic acids, genomic SELEX involves iterative binding, partitioning, and amplification of nucleic acids. The two methods differ in that the variable region of the nucleic acid library for genomic SELEX is derived from the genome of an organism. We have used a quick and simple method to construct Escherichia coli, Saccharomyces cerevisiae, and human genomic DNA PCR libraries that can be transcribed with T7 RNA polymerase. We present evidence that the libraries contain overlapping inserts starting at most of the positions within the genome, making these libraries suitable for genomic SELEX.
Singer BS, Shtatland T, Brown D, Gold L
Nucleic acids research
1997-02-15 00:00
25
4
781-6
Binding Sites,DNA, Bacterial,DNA, Fungal,Escherichia coli,Gene Dosage,Genomic Library,Humans,Nucleic Acids,Placenta,Polymerase Chain Reaction,Protein Binding,Saccharomyces cerevisiae,DNA, Bacterial,DNA, Fungal,Nucleic Acids
Department of Molecular Biology, University of Colorado, Boulder 80309-0347, USA
Nucleic Acids Res.
NIGMS GM19963
0305-1048
gka142
420
True
9016629
