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In vitro selection of self-cleaving RNAs with a low pH optimum
In vitro selection of self-cleaving RNAs with a low pH optimum.
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RNAs that undergo a rapid site-specific cleavage at low pH have been selected by in vitro selection (the SELEX process). The cleavage does not require the addition of any divalent metal ions, and is in fact inhibited by divalent metal ions, spermine, or high concentrations of monovalent metal ions. This low pH catalyzed cleavage results in a 2',3'-cyclic phosphate at the 3' end and a free hydroxyl at the 5' end. The reaction proceeds with a calculated rate of 1.1 min-1 at room temperature in cacodylate buffer at pH 5.0. The rate of cleavage is dependent on the pH and shows an optimum around pH 4.0. The rate constant is independent of RNA concentration, indicating to an intramolecular reaction. Autocatalytic cleavage at low pH, in the absence of a metal ion requirement, adds to the reaction possibilities that may have existed on the prebiotic earth.
Jayasena VK, Gold L
Proceedings of the National Academy of Sciences of the United States of America
1997-09-30 00:00
94
20
10612-7
Catalysis,Hydrogen-Ion Concentration,Hydrolysis,Metals,RNA, Catalytic,Metals,RNA, Catalytic
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM19963
0027-8424
413
True
9380683
