Document Actions
High affinity ligands from in vitro selection complex targets
High affinity ligands from in vitro selection: complex targets.
54
Human red blood cell membranes were used as a model system to determine if the systematic evolution of ligands by exponential enrichment (SELEX) methodology, an in vitro protocol for isolating high-affinity oligonucleotides that bind specifically to virtually any single protein, could be used with a complex mixture of potential targets. Ligands to multiple targets were generated simultaneously during the selection process, and the binding affinities of these ligands for their targets are comparable to those found in similar experiments against pure targets. A secondary selection scheme, deconvolution-SELEX, facilitates rapid isolation of the ligands to targets of special interest within the mixture. SELEX provides high-affinity compounds for multiple targets in a mixture and might allow a means for dissecting complex biological systems.
Morris KN, Jensen KB, Julin CM, Weil M, Gold L
Proceedings of the National Academy of Sciences of the United States of America
1998-03-17 00:00
95
6
2902-7
Blood Proteins,Cloning, Molecular,DNA, Single-Stranded,Erythrocyte Membrane,Evolution, Molecular,Ligands,Membrane Proteins,Oligodeoxyribonucleotides,Selection (Genetics),Blood Proteins,DNA, Single-Stranded,Ligands,Membrane Proteins,Oligodeoxyribonucleotides
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM 19963, NIGMS GM 28685
0027-8424
404
True
9501188
