Document Actions
From oligonucleotide shapes to genomic SELEX novel biological regulatory loops
From oligonucleotide shapes to genomic SELEX: novel biological regulatory loops.
54
The SELEX method and oligonucleotide combinatorial chemistry discovery process yields high-affinity/high-specificity ligands for virtually any molecular target. Typically, the enormous starting libraries used in the SELEX process contain 10(14)-10(15) sequences. We now ask if the smaller sequences, complexity of extant organisms, and evolutionary history provide useful interactions between oligonucleotides and at least some unexpected targets. That is, do organisms contain a robust linkage map between their oligonucleotides and proteins and/or small molecules that enriches life?
Gold L, Brown D, He Y, Shtatland T, Singer BS, Wu Y
Proceedings of the National Academy of Sciences of the United States of America
1997-01-07 00:00
94
1
59-64
Base Sequence,Binding Sites,DNA-Directed DNA Polymerase,Evolution, Molecular,Gene Expression Regulation,Ligands,Molecular Biology,Molecular Sequence Data,Nucleic Acid Conformation,Oligoribonucleotides,RNA,Viral Proteins,Ligands,Oligoribonucleotides,Viral Proteins,gene 43 protein, Enterobacteria phage T4,RNA,DNA-Directed DNA Polymerase
University of Colorado at Boulder, Department of Molecular, Cellular, and Developmental Biology, 80309-0347, USA
Proc. Natl. Acad. Sci. U.S.A.
0027-8424
402
True
8990161
