Document Actions
Domains of initiator tRNA and initiation ... lection by Escherichia coli IF3
Domains of initiator tRNA and initiation codon crucial for initiator tRNA selection by Escherichia coli IF3.
54
Initiation factors are used by Escherichia coli to select the initiator tRNA over elongator tRNAs during translation initiation. IF3 appears to inspect the anticodon end of the tRNA, probably along with the initiation codon. The anticodon stem and loop of the initiator tRNA, together with part of the initiation codon of the mRNA, can be thought of as a unit. Changes made in the anticodon stem, the anticodon loop, or the anticodon of an initiator tRNA fragment result in a loss of selection by IF3 in an in vitro assay for translation initiation. IF3 allows the selection of an initiator tRNA anticodon stem and loop fragment on GUG and UUG codons but does not select that tRNA fragment in response to AUU.
Hartz D, Binkley J, Hollingsworth T, Gold L
Genes & development
1990-10-01 00:00
4
10
1790-800
Bacterial Proteins,Base Sequence,Codon,Escherichia coli,Molecular Sequence Data,Nucleic Acid Conformation,Peptide Chain Initiation, Translational,Peptide Initiation Factors,Prokaryotic Initiation Factor-3,RNA, Bacterial,RNA, Fungal,RNA, Transfer,Saccharomyces cerevisiae,Triticum,Bacterial Proteins,Codon,Peptide Initiation Factors,Prokaryotic Initiation Factor-3,RNA, Bacterial,RNA, Fungal,RNA, Transfer
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309
Genes Dev.
NIGMS GM-28685
0890-9369
397
True
1701151
