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A tenascin-C aptamer identified by tumor ... gands by exponential enrichment
A tenascin-C aptamer identified by tumor cell SELEX: systematic evolution of ligands by exponential enrichment.
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The targeting of molecular repertoires to complex systems rather than biochemically pure entities is an accessible approach that can identify proteins of biological interest. We have probed antigens presented by a monolayer of tumor cells for their ability to interact with a pool of aptamers. A glioblastoma-derived cell line, U251, was used as the target for systematic evolution of ligands by exponential enrichment by using a single-stranded DNA library. We isolated specifically interacting oligonucleotides, and biochemical strategies were used to identify the protein target for one of the aptamers. Here we characterize the interaction of the DNA aptamer, GBI-10, with tenascin-C, an extracellular protein found in the tumor matrix. Tenascin-C is believed to be involved in both embryogenesis and oncogenesis pathways. Systematic evolution of ligands by exponential enrichment appears to be a successful strategy for the a priori identification of targets of biological interest within complex systems.
Daniels DA, Chen H, Hicke BJ, Swiderek KM, Gold L
Proceedings of the National Academy of Sciences of the United States of America
2003-12-23 00:00
100
26
15416-21
Amino Acid Sequence,Base Sequence,Chromatography, Liquid,DNA, Single-Stranded,Enzyme-Linked Immunosorbent Assay,Evolution,Glioblastoma,Humans,Ligands,Mass Spectrometry,Membrane Proteins,Molecular Sequence Data,Oligodeoxyribonucleotides,Peptide Fragments,Templates, Genetic,Tenascin,Tumor Cells, Cultured,DNA, Single-Stranded,Ligands,Membrane Proteins,Oligodeoxyribonucleotides,Peptide Fragments,Tenascin
Gene Expression and Protein Biochemistry, GlaxoSmithKline Medicines Research Centre, Stevenage, Hertfordshire SG1 2NY, England
Proc. Natl. Acad. Sci. U.S.A.
0027-8424
10.1073/pnas.2136683100
2136683100
386
True
14676325
