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Gene-specific requirement for P-TEFb act ... the p53 transcriptional program
Gene-specific requirement for P-TEFb activity and RNA polymerase II phosphorylation within the p53 transcriptional program.
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Activation of the p53 pathway mediates cellular responses to diverse forms of stress. Here we report that the p53 target gene p21(CIP1) is regulated by stress at post-initiation steps through conversion of paused RNA polymerase II (RNAP II) into an elongating form. High-resolution chromatin immunoprecipitation assays (ChIP) demonstrate that p53-dependent activation of p21(CIP1) transcription after DNA damage occurs concomitantly with changes in RNAP II phosphorylation status and recruitment of the elongation factors DSIF (DRB Sensitivity-Inducing Factor), P-TEFb (Positive Transcription Elongation Factor b), TFIIH, TFIIF, and FACT (Facilitates Chromatin Transcription) to distinct regions of the p21(CIP1) locus. Paradoxically, pharmacological inhibition of P-TEFb leads to global inhibition of mRNA synthesis but activation of the p53 pathway through p53 accumulation, expression of specific p53 target genes, and p53-dependent apoptosis. ChIP analyses of p21(CIP1) activation in the absence of functional P-TEFb reveals the existence of two distinct kinases that phosphorylate Ser5 of the RNAP II C-terminal domain (CTD). Importantly, CTD phosphorylation at Ser2 is not required for p21(CIP1) transcription, mRNA cleavage, or polyadenylation. Furthermore, recruitment of FACT requires CTD kinases, yet FACT is dispensable for p21(CIP1) expression. Thus, select genes within the p53 pathway bypass the requirement for P-TEFb and RNAP II phosphorylation to trigger a cellular response to inhibition of global mRNA synthesis.
Gomes NP, Bjerke G, Llorente B, Szostek SA, Emerson BM, Espinosa JM
Genes & development
2006-03-01 00:00
20
5
601-12
Apoptosis,Chromatin Immunoprecipitation,Cyclin-Dependent Kinase Inhibitor p21,DNA,DNA-Binding Proteins,Doxorubicin,Flow Cytometry,HCT116 Cells,High Mobility Group Proteins,Humans,Kinetics,Nuclear Proteins,Phosphorylation,Positive Transcriptional Elongation Factor B,Protein Isoforms,Protein Structure, Tertiary,RNA Polymerase II,Reverse Transcriptase Polymerase Chain Reaction,Serine,Transcription Factor TFIIH,Transcription Factors,Transcription Factors, TFII,Transcription, Genetic,Transcriptional Elongation Factors,Tumor Suppressor Protein p53,Cyclin-Dependent Kinase Inhibitor p21,DNA-Binding Proteins,DSIF protein, human,High Mobility Group Proteins,Nuclear Proteins,Protein Isoforms,SSRP1 protein, human,Transcription Factors,Transcription Factors, TFII,Transcriptional Elongation Factors,Tumor Suppressor Protein p53,transcription factor TFIIF,Transcription Factor TFIIH,Doxorubicin,Serine,DNA,Positive Transcriptional Elongation Factor B,RNA Polymerase II
Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA
Genes Dev.
NCI CA054418, NIGMS GM07135
0890-9369
10.1101/gad.1398206
20/5/601
379
True
16510875
