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An early ancestor in the evolution of sp ... R is functional in cis-splicing
An early ancestor in the evolution of splicing: a Trypanosoma cruzi serine-arginine-rich protein (TcSR) is functional in cis-splicing.
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A novel serine-arginine-rich protein designated TcSR was identified in Trypanosoma cruzi. The deduced amino acid sequence reveals that TcSR is a member of the SR protein family of splicing factors that contains two RNA-binding domains at the N-terminal side and several serine-arginine repeats at the COOH-terminus. Over expression of either TcSR or the human SR-protein associated splicing factor/splicing factor 2 (ASF/SF2) in wild-type Schizosaccharomyces pombe, provoked an elongated phenotype similar to that of fission yeast over expressing the SR-containing splicing factor Prp2, a U2AF(65) orthologue. When a double mutant strain lacking two SR protein-specific protein kinases was used, expression of TcSR or human SR ASF/SF2 splicing factor reverted the mutant to a wild-type phenotype. Transient expression of TcSR in HeLa cells stimulated the inclusion of the EDI exon of human fibronectin in an in vivo functional alternative cis-splicing assay. Inclusion was dependent on a splicing enhancer sequence present in the EDI exon. In addition, TcSR and peptides carrying TcSR-RS domain sequences were phosphorylated by a human SR protein kinase. These results indicate that TcSR is a member of the SR splicing network and that some components common to the trans- and cis-splicing machineries evolved from the early origins of the eukaryotic lineage.
Portal D, Espinosa JM, Lobo GS, Kadener S, Pereira CA, De La Mata M, Tang Z, Lin RJ, Kornblihtt AR, Baralle FE, Flawiá MM, Torres HN
Molecular and biochemical parasitology
2003-03-01 00:00
127
1
37-46
Amino Acid Sequence,Animals,Arginine,Evolution, Molecular,Hela Cells,Humans,Protozoan Proteins,RNA Splicing,Schizosaccharomyces,Sequence Alignment,Serine,Trypanosoma cruzi,Protozoan Proteins,Serine,Arginine
Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones en Ingeniera Gentica y Biologa Molecular, Consejo Nacional de Investigaciones Cientficas y Tcnicas, Universidad de Buenos Aires, Buenos Aires, Argentina
Mol. Biochem. Parasitol.
0166-6851
S0166685102003018
377
True
12615334
