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Host microarray analysis reveals a role ... in human macrophage cell death
Host microarray analysis reveals a role for the Salmonella response regulator phoP in human macrophage cell death.
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Bacterial pathogens manipulate host cells to promote pathogen survival and dissemination. We used a 22,571 human cDNA microarray to identify host pathways that are affected by the Salmonella enterica subspecies typhimurium phoP gene, a transcription factor required for virulence, by comparing the expression profiles of human monocytic tissue culture cells infected with either the wild-type bacteria or a phoPTn10 mutant strain. Both wild-type and phoPTn10 bacteria induced a common set of genes, many of which are proinflammatory. Differentially expressed genes included those that affect host cell death, suggesting that the phoP regulatory system controls bacterial genes that alter macrophage survival. Subsequent experiments showed that the phoPTn10 mutant strain is defective for killing both cultured and primary human macrophages but is able to replicate intracellularly. These experiments indicate that phoP plays a role in Salmonella-induced human macrophage cell death.
Detweiler CS, Cunanan DB, Falkow S
Proceedings of the National Academy of Sciences of the United States of America
2001-05-08 00:00
98
10
5850-5
Bacterial Proteins,Blotting, Northern,Cell Death,DNA, Complementary,Genes, cdc,Humans,Macrophages,Mutation,Oligonucleotide Array Sequence Analysis,RNA, Messenger,Salmonella typhimurium,Transcription Factors,Bacterial Proteins,DNA, Complementary,RNA, Messenger,Transcription Factors,PhoP protein, Bacteria
Department of Microbiology and Immunology, Stanford University Medical Center, Stanford, CA 94305, USA corriedlelandstanfordedu
Proc. Natl. Acad. Sci. U.S.A.
NIAID AI26195
0027-8424
10.1073/pnas.091110098
091110098
354
True
11320214
