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Two modes of survival of fission yeast without telomerase
Two modes of survival of fission yeast without telomerase.
68
Deletion of the telomerase catalytic subunit gene trt1+ in Schizosaccharomyces pombe results in death for the majority of cells, but a subpopulation survives. Here it is shown that most survivors have circularized all of their chromosomes, whereas a smaller number maintain their telomeres presumably through recombination. When the telomeric DNA-binding gene taz1+ is also deleted, trt1- taz1- survivors use the recombinational mode more frequently. Moreover, the massive elongation of telomeres in taz1- cells is absent in the double mutant. Thus, Taz1p appears to regulate telomeric recombination as well as telomerase activity in fission yeast.
Nakamura TM, Cooper JP, Cech TR
Science (New York, N.Y.)
1998-10-16 00:00
282
5388
493-6
Chromosomes, Fungal,DNA Probes,DNA, Fungal,DNA-Binding Proteins,Gene Deletion,Genes, Fungal,Proteins,RNA,Recombination, Genetic,Schizosaccharomyces,Schizosaccharomyces pombe Proteins,Telomerase,Telomere,Telomere-Binding Proteins,DNA Probes,DNA, Fungal,DNA-Binding Proteins,Proteins,Schizosaccharomyces pombe Proteins,Telomere-Binding Proteins,taz1 protein, S pombe,telomerase RNA,trt1 protein, S pombe,RNA,Telomerase
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA
Science
NIGMS GM28039
0036-8075
312
True
9774280
