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Structure of human POT1 bound to telomer ... l for chromosome end-protection
Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection.
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The POT1 (protection of telomeres 1) protein binds the single-stranded overhang at the ends of chromosomes in diverse eukaryotes. It is essential for chromosome end-protection in the fission yeast Schizosaccharomyces pombe, and it is involved in regulation of telomere length in human cells. Here, we report the crystal structure at a resolution of 1.73 A of the N-terminal half of human POT1 (hPOT1) protein bound to a telomeric single-stranded DNA (ssDNA) decamer, TTAGGGTTAG, the minimum tight-binding sequence indicated by in vitro binding assays. The structure reveals that hPOT1 contains two oligonucleotide/ oligosaccharide-binding (OB) folds; the N-terminal OB fold binds the first six nucleotides, resembling the structure of the S. pombe Pot1pN-ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic-resolution model for chromosome end-capping.
Lei M, Podell ER, Cech TR
Nature structural & molecular biology
2004-12-01 00:00
11
12
1223-9
Base Sequence,Crystallography, X-Ray,DNA, Single-Stranded,Humans,Models, Biological,Models, Molecular,Nucleic Acid Conformation,Protein Binding,Protein Structure, Tertiary,Schizosaccharomyces pombe Proteins,Telomerase,Telomere,Telomere-Binding Proteins,DNA, Single-Stranded,POT1 protein, human,Schizosaccharomyces pombe Proteins,Telomere-Binding Proteins,pot1 protein, S pombe,Telomerase
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA
Nat. Struct. Mol. Biol.
1545-9993
10.1038/nsmb867
nsmb867
277
True
15558049
