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A quantitative study of the flexibility ... nicks and single-stranded gaps
A quantitative study of the flexibility contributed to RNA structures by nicks and single-stranded gaps.
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Disulfide crosslinking via thiol-disulfide interchange was applied to quantitate the relative flexibility contributed by nicks and single-stranded gaps in an RNA structure. An RNA duplex comprised of three strands was constructed containing the disulfide crosslink precursors 1 and 2 at opposite ends of the duplex on opposite strands. The third strand was of varying length to yield a nick or single-stranded gaps of 1, 2, or 3 nt. Crosslinking rates Indicated relative flexibilities of the resulting two-helix junctions. Crosslinking in the nicked duplex occurred two orders of magnitude slower than in a duplex containing a 3-nt gap. Rates of crosslinking in duplexes with 3-and 2-nt gaps showed only modest dependence on the gap sequence. Many natural RNAs, including ribozymes, contain two-helix junctions related to the model system described here. The data suggest that two-helix junctions containing a nick in one strand will retain substantial rigidity, whereas one or more single-stranded nucleotides at a two-helix junction allow significant flexibility.
Cohen SB, Cech TR
RNA (New York, N.Y.)
1998-10-01 00:00
4
10
1179-85
Cross-Linking Reagents,Disulfides,Nucleic Acid Conformation,Oligoribonucleotides,RNA,RNA, Double-Stranded,Cross-Linking Reagents,Disulfides,Oligoribonucleotides,RNA, Double-Stranded,RNA
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215, USA
RNA
1355-8382
133
True
9769093
