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trans-spliced Caenorhabditis elegans mRNAs retain trimethylguanosine caps
trans-spliced Caenorhabditis elegans mRNAs retain trimethylguanosine caps.
89
The nematode Caenorhabditis elegans has an unusual small nuclear RNA, containing a 100-nucleotide RNA molecule, spliced leader RNA, which donates its 5' 22 nucleotides to a variety of recipient RNAs by a trans-splicing reaction. The spliced leader RNA has a 5' trimethylguanosine (TMG) cap, which becomes the 5' end of trans-spliced mRNAs. We found that mature trans-spliced mRNAs were immunoprecipitable with anti-TMG cap antibodies and that TMG-containing dinucleotides specifically competed with the trans-spliced mRNAs for antibody binding. We also found that these mRNAs retained their TMG caps throughout development and that the TMG-capped mRNAs were polysome associated. Since the large majority of C. elegans mRNAs are not trans-spliced, the addition of the spliced leader and its TMG cap to a limited group of recipient RNAs may create a functionally distinct subset of mRNAs.
Liou RF, Blumenthal T
Molecular and cellular biology
1990-04-01 00:00
10
4
1764-8
Actins,Animals,Caenorhabditis,Endoribonucleases,Guanosine,Immunoassay,Oligonucleotide Probes,Polyribosomes,RNA Cap Analogs,RNA Caps,RNA Splicing,RNA, Messenger,Ribonuclease H, Calf Thymus,Actins,Oligonucleotide Probes,RNA Cap Analogs,RNA Caps,RNA, Messenger,Guanosine,N(2),N(2),7-trimethylguanosine,Endoribonucleases,Ribonuclease H, Calf Thymus
Program in Molecular, Cellular and Developmental Biology, Indiana University, Bloomington 47405
Mol. Cell. Biol.
0270-7306
85
True
2157142
