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SL1 trans-splicing specified by AU-rich ... Caenorhabditis elegans pre-mRNA
SL1 trans-splicing specified by AU-rich synthetic RNA inserted at the 5' end of Caenorhabditis elegans pre-mRNA.
89
In Caenorhabditis elegans, pre-mRNAs of many genes are trans-spliced to one of two spliced leaders, SL1 or SL2. Some of those that receive exclusively SL1 have been characterized as having at their 5' ends outrons, AU-rich sequences similar to introns followed by conventional 3' splice sites. Comparison of outrons from many different SL1-specific C. elegans genes has not revealed the presence of any consensus sequence that might encode SL1-specificity. In order to determine what parameters influence the splicing of SL1, we performed in vivo experiments with synthetic splice sites. Synthetic AU-rich RNA, 51 nt or longer, placed upstream of a consensus 3' splice site resulted in efficient trans-splicing. With all sequences tested, this trans-splicing was specifically to SL1. Thus, no information beyond the presence of AU-rich RNA at least as long as the minimum-length C. elegans intron, followed by a 3' splice site, is required to specify trans-splicing or for strict SL1 specificity.
Conrad R, Lea K, Blumenthal T
RNA (New York, N.Y.)
1995-04-01 00:00
1
2
164-70
Adenine,Animals,Base Sequence,Binding Sites,Caenorhabditis elegans,DNA Primers,Genes, Helminth,Helminth Proteins,Introns,Molecular Sequence Data,Nucleic Acid Conformation,RNA Precursors,RNA Splicing,RNA, Helminth,RNA, Messenger,Uracil,DNA Primers,Helminth Proteins,RNA Precursors,RNA, Helminth,RNA, Messenger,Uracil,Adenine
Department of Biology, Indiana University, Bloomington 47405, USA
RNA
NIGMS GM42432
1355-8382
69
True
7585246
