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Q beta replicase containing a Bacillus stearothermophilus elongation factor
Q beta replicase containing a Bacillus stearothermophilus elongation factor.
89
We purified Q beta replicase containing EF-Ts from Bacillus stearothermophilus in place of the homologous polypeptide from Escherichia coli. The hybrid enzyme was fully active in the transcription of a variety of templates. It was found to be qualitatively similar to native Q beta replicase with respect to a variety of parameters which measure the efficiency of initiation of RNA synthesis. The results demonstrated that Q beta replicase can tolerate substantial alterations in the EF-Tu X Ts component of the enzyme. These alterations resulted in only minor perturbations of catalytic properties.
Stringfellow L, Blumenthal T
Journal of bacteriology
1983-02-01 00:00
153
2
1083-7
Bacillus stearothermophilus,Escherichia coli,Guanosine Diphosphate,Guanosine Triphosphate,Peptide Elongation Factor Tu,Peptide Elongation Factors,Q beta Replicase,RNA Nucleotidyltransferases,RNA, Viral,Peptide Elongation Factors,Qbeta ribonucleic acid,RNA, Viral,Guanosine Diphosphate,Guanosine Triphosphate,Q beta Replicase,RNA Nucleotidyltransferases,Peptide Elongation Factor Tu
J. Bacteriol.
NIGMS GM21024
0021-9193
59
True
6337116
