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Alteration of Caenorhabditis elegans gene expression by targeted transformation
Alteration of Caenorhabditis elegans gene expression by targeted transformation.
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We have produced strains carrying a synthetic fusion of parts of two vitellogenin genes, vit-2 and vit-6, integrated into the Caenorhabditis elegans genome. In most of the 63 transformant strains, the plasmid sequences are integrated at random locations in the genome. However, in two strains the transgene integrated by homologous recombination into the endogenous vit-2 gene. In both cases the reciprocal exchange between the chromosome and the injected circular plasmid containing a promoter deletion led to switching of the plasmid-borne promoter and the endogenous promoter, with a reduction in vit-2 expression. Thus in nematodes, transforming DNA can integrate by homologous recombination to result in partial inactivation of the chromosomal locus. The simplicity of the event and its reasonably high frequency suggest that gene targeting by homologous recombination should be considered as a method for directed inactivation of C. elegans genes.
Broverman S, MacMorris M, Blumenthal T
Proceedings of the National Academy of Sciences of the United States of America
1993-05-15 00:00
90
10
4359-63
Animals,Caenorhabditis,Chromosome Mapping,Gene Expression Regulation,Genes, Helminth,In Situ Hybridization,Mutagenesis, Insertional,Promoter Regions (Genetics),RNA, Messenger,Recombination, Genetic,Transfection,Vitellogenins,RNA, Messenger,Vitellogenins
Department of Biology, Indiana University, Bloomington 47405
Proc. Natl. Acad. Sci. U.S.A.
NIGMS GM07227, NIGMS GM30870
0027-8424
6
True
8506273
